RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae

Table 3 Effect of bmugm gene silencing on viability of adult B. malayi female parasites assessed by motility scoring and MTT reduction assay

Time (h)	Motility score (female worms)^a					% inhibition in MTT reduction (mean ± SD)
Time (h)	MC	SCR	siRNA 1	siRNA 2	siRNA 1 + 2	SCR	siRNA 1	siRNA 2	siRNA 1 + 2
24	5+	5+	2+	2+	2+	5.2 ± 0.40	66.1 ± 5.58**	57.9 ± 0.80**	58.8 ± 9.89**
48	5+	4+	1+	1+	1+	3.7 ± 3.40	86.9 ± 1.70***	83.4 ± 3.67***	87.6 ± 1.9***
72	4+	4+	1+	1+	1+	11.1 ± 1.84	87.3 ± 9.98***	88.6 ± 1.58***	98.1 ± 2.26***
96	4+	4+	1+	1+	1+	11.3 ± 3.17	88.7 ± 2.47***	91.5 ± 1.93***	92.2 ± 2.64***
120	4+	4+	1+	1+	1+	5.3 ± 0.83	87.8 ± 3.9***	87.4 ± 2.8***	90.1 ± 3.73***

Abbreviations: MC medium control; MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
**P < 0.01; ***P < 0.001
^a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction

ISSN: 1756-3305