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Table 3 Effect of bmugm gene silencing on viability of adult B. malayi female parasites assessed by motility scoring and MTT reduction assay

From: RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae

Time (h) Motility score (female worms)a % inhibition in MTT reduction (mean ± SD)
MC SCR siRNA 1 siRNA 2 siRNA 1 + 2 SCR siRNA 1 siRNA 2 siRNA 1 + 2
24 5+ 5+ 2+ 2+ 2+ 5.2 ± 0.40 66.1 ± 5.58** 57.9 ± 0.80** 58.8 ± 9.89**
48 5+ 4+ 1+ 1+ 1+ 3.7 ± 3.40 86.9 ± 1.70*** 83.4 ± 3.67*** 87.6 ± 1.9***
72 4+ 4+ 1+ 1+ 1+ 11.1 ± 1.84 87.3 ± 9.98*** 88.6 ± 1.58*** 98.1 ± 2.26***
96 4+ 4+ 1+ 1+ 1+ 11.3 ± 3.17 88.7 ± 2.47*** 91.5 ± 1.93*** 92.2 ± 2.64***
120 4+ 4+ 1+ 1+ 1+ 5.3 ± 0.83 87.8 ± 3.9*** 87.4 ± 2.8*** 90.1 ± 3.73***
  1. Abbreviations: MC medium control; MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
  2. **P < 0.01; ***P < 0.001
  3. a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction