Table 2 Effects of different experimental parameters on the engorgement and fecundity of artificially fed Dermacentor reticulatus females
From: Optimization of an artificial tick feeding assay for Dermacentor reticulatus
Experiment | Experimental group | Engorgement rate (%) | Mean engorgement mass ± SD (mg) | Females producing fertile eggs (%) | Mean egg batch mass ± SD (mg) |
|---|---|---|---|---|---|
I | Defrosted blood | 37.8 (45/119) | 198.3 ± 63.7 | 71.1 (32/45) | 95.0 ± 37.7 |
Fresh blood | 49.6 (59/119) | 221.9 ± 63.2 | 71.2 (42/59) | 98.3 ± 38.9 | |
II | Untreated blood | 32.0 (47/147) | 222.9 ± 78.1 | 53.2 (25/47)a | 74.4 ± 53.3c |
Irradiated blood | 34.0 (50/147) | 193.7 ± 64.4 | 56.0 (28/50)b | 40.4 ± 29.3cd | |
Gentamycin-treated blood | 29.3 (43/147) | 215.6 ± 78.3 | 79.1 (34/43)ab | 94.2 ± 41.8d | |
III | Ambient CO2 | 17.0 (31/182) | 220.3 ± 101.3e | 32.3 (10/31)f | 107 ± 60.1g |
5% CO2 | 25.3 (46/182) | 303.9 ± 105.1e | 82.6 (38/46)f | 178.8 ± 86.7g | |
IV | 2 g/l Glucose, 2.5% CO2 | 31.2 (24/77) | 259.5 ± 71.9 | 87.5 (21/24) | 99.8 ± 38.9 |
4 g/l Glucose, 2.5% CO2 | 42.9 (33/77) | 287.5 ± 68.8 | 87.9 (29/33) | 121.3 ± 70.4 | |
Ticks fed on rabbits | 64 (64/100) | 266.3 ± 71.4 | 100 (64/64) | 123.9 ± 54.3 |