PCR protocol | No. of positive samples (%) |
---|
CS dogs (n = 150) | AH dogs (n = 79) | All dogs (n = 229) |
---|
Nested SSU rRNA-PCR | 66 (44.0)a
| 20 (25.3)a
| 86 (37.6)d,e,f
|
ITS1-PCR | 18 (12.0)b
| 2 (1.3)b
| 20 (8.7)d
|
MC-PCR | 28 (18.7)c
| 5 (6.3)c
| 33 (14.4)e
|
Uni21/Lmj4-PCR | 23 (15.3) | 10 (12.7) | 33 (14.4)f
|
-
Abbreviations: AH apparently healthy, CS clinically suspected, ITS1 internal transcribed spacer 1, MC minicircle, PCR polymerase chain reaction, rRNA ribosomal RNA gene, SSU small subunit, Uni21/Lmj4 Uni21 primer based on a conserved region of a Leishmania major kinetoplastid minicircle sequence, and Lmj4 based on the variable region of the same L. major sequence
- Only statistically significant differences are shown: a
Z = 2.76, P = 0.006; b
Z = 2.41, P = 0.016; c
Z = 2.53, P = 0.012; d
Z = 7.31, P < 0.001; e,f
Z = 5.65, P < 0.001