Fig. 5

Proliferation analysis of splenocytes from immunized mice in vitro. Splenocytes from ACT-F- high dosage, ACT-T- high dosage, FA-sham (PBS in adjuvant) and unimmunized groups were stimulated with ACT-F or ACT-T (10 μg/ml) for 68 h in vitro. Proliferation was determined using CCK8 cell counting kits. The stimulation index (SI) is calculated as the ratio of the proliferation of stimulated cells to non-stimulated cells in the same group. The data are the mean SI ± SEM from three individual mice from each group with three repeats (n = 3). Significance was accepted by comparison with the unimmunized (*P < 0.05; **P < 0.01; ***P < 0.001) and FA-sham groups (^# P < 0.05; ^## P < 0.01; ^### P < 0.001) using statistical analyses