Fig. 9

Cytokines secretion by BMDCs induced by CsHSP/ProR peptide complexes. rCsHSP70 or rCsHSP90 (1 μg/ml) was mixed with ProR peptide at a 200:1 peptide:protein molar ratio in binding buffer at 37 °C for 1 h. After polymyxin B treatment for 1 h, BMDCs were treated with rCsHSP70, rCsHSP90, CsProR peptide or CsHSP/ProR complexes. Levels of secreted cytokines were measured by ELISA. Panel a shows results by CsHSP70/CsProR and panel b shows results by CsHSP90/CsProR. The results are shown as the mean ± standard error (SEM). *P < 0.05, **P < 0.01, ***P < 0.001