Fig. 1
From: Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti
Whole body gene expression of Toll and IMD pathway genes are induced upon blood-feeding. Gene expression of the three main mosquito immune pathways was evaluated by qPCR using whole body samples 24 h post blood-feeding. a Toll pathway components. b IMD pathway components. c Jak/STAT pathway components. The blood-fed samples were compared with sugar fed samples. Relative expression was calculated using the ΔΔCt method using sugar fed expression as reference. RP49 gene was used as a reference gene. We performed Student’s t-test for statistical analysis between sugar and blood-fed conditions for each gene analyzed