Fig. 4

Microbiota depletion impacts aaREL2 expression in response to SINV infection. Relative gene expression of the transcription factors of Toll, IMD and Jak/STAT pathways was evaluated in response to microbiota depletion and SINV infection. Mosquitoes were either fed with blood, pretreated for 3 days with a sugar solution containing antibiotic (penicillin and streptomycin) and after that fed with blood or infected blood containing SINV particles. a Relative gene expression of aaREL1 4 days post-infection. b Relative gene expression of aaREL2 4 days post-infection. c Relative gene expression of aaSTAT 4 days post-infection. Relative expression was calculated using the ΔΔCt method and setting the blood condition as reference for comparison. RP49 was used as a reference gene. We performed One-way ANOVA Tukey multiple comparison test for statistical analyses