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Table 1 Frequency of microscopic lesions and detection of Leishmania by means of HE, IHC and qPCR on paired skin samples from the dogs studied based on disease stage. Values with the same superscript differ significantly

From: Histological and parasitological distinctive findings in clinically-lesioned and normal-looking skin of dogs with different clinical stages of leishmaniosis

Skin samples Microscopic lesions Detection of Leishmania
HE IHQ qPCR
Normal-looking skin (n = 25) 14/25 (56.0 %) 5/25 (20%) 8/25 (32.0%) 18/25 (72.0%)
 Stage I (n = 11) 3/11 (27.3%)a,b 0/11 (0%)c 1/11 (9.1%)d 5/11 (45.5%)e
 Stage II-III (n = 14) 11/14 (78.6%)b 5/14 (35.7%)c 7/14 (50.0%)d 13/14 (92.9%)e
Clinically-lesioned skin (n = 25) 25/25 (100%) 11/25 (44.0%) 23/25 (92.0%) 25/25 (100%)
 Stage I (n = 11) 11/11 (100%)a 1/11 (9.1%)f 9/11 (81.8%) 11/11 (100%)
 Stage II-III (n = 14) 14/14 (100%) 10/14 (71.4%)f 14/14 (100%) 14/14 (100%)
  1. Abbreviations: HE haematoxylin and eosin stained sections, IHC Leishmania immunohistochemistry, qPCR quantitative PCR
  2. aMcNemar's test: P = 0.008
  3. bFisher’s exact test: P = 0.0172
  4. cFisher’s exact test: P = 0.0464
  5. dFisher’s exact test: P = 0.0421
  6. eFisher’s exact test: P = 0.0068
  7. fFisher’s exact test: P = 0.0037