Table 1 Polymerase chain reaction details for the qPCR/PCR assays used in the study for the testing of infectious agents
Target species (target gene) | PCR primer or probe sequences (5'–3') | Product size (bp) | Reference |
|---|---|---|---|
Bartonella henselae (alr-gcvP intergenic spacer) | F: GAGGGAAATGACTCTCTCAGTAAAA | 110 | [20]a |
R: TGAACAGGATGTGGAAGAAGG | |||
FAM-CAGCCAAATATACGGGCTATCCATCAA-TAMRA | |||
“Candidatus Mycoplasma haemominutum” (16S rRNA gene) | F: TGATCTATTGTKAAAGGCACTTGCT | 135 | [18] |
R: TTAGCCTCYGGTGTTCCTCAA | |||
FAM-TTCAATGTGTAGCGGTGGAATGCGT-BHQ1 | |||
“Candidatus Mycoplasma turicensis” (16S rRNA gene) | F: AGAGGCGAAGGCGAAAACT | 138 | [18] |
R: ACGTAAGCTACAACGCCGAAA | |||
FAM-CGTAAACGATGGGTATTAGATGTCGGGAT-BHQ1 | |||
Ehrlichia/Anaplasma spp. (16S rRNA gene) | F: GGTACCYACAGAAGAAGTCC | 345 | [21] |
R: TAGCACTCATCGTTTACAGC | |||
Hepatozoon. spp. (18S rRNA gene) | F: AAACGGCTACCACATNTAAGGA | 522 | |
R: AATACAAATGCCCCCAACTNT | |||
Leishmania spp. (screening assay) (kinetoplast DNA, kDNA) | F: CGGGTAGGGGCGTTCTG | 115 | [19] |
R: ATTTTACACCAACCCCCAGTT | |||
FAM-TGGGTGCAGAAATCCCGTTCA-BHQ1 | |||
Leishmania spp. (confirmatory assay) (kDNA) | F: CCTATTTTACACCAACCCCCAGT | 120 | |
R: GGGTAGGGGCGTTCTGCGAAA | |||
Mycoplasma haemofelis (16S rRNA gene) | F: GTGCTACAATGGCGAACACA | 80 | [18] |
R: TCCTATCCGAACTGAGACGAA | |||
FAM-TGTGTTGCAAACCAGCGATGGT-BHQ1 |