Table 2 Primer combinations and concentrations used for species of the genus Babesia and species-specific (sp-sp) PCRs

Babesia genus LSU qPCR

B-lsu-F (0.6); B-lsu-R2 (0.6); Bmic-F (0.4)

Babesia genus 18S qPCR

Bcommon-F (0.4); Bcommon-R (0.4)

B. microti-like sp-sp 18S qPCR

BMIC18-F (0.8); BAB722 (0.8)

B. vogeli sp-sp 18S qPCR

BCV-F (0.4); BAB722 (0.4)

B. canis sp-sp 18S qPCR

BCC-F (0.4); BAB722 (0.4)

B. coco sp-sp 18S qPCR

BCO-F (0.4); BAB722 (0.4)

B. gibsoni sp-sp 18S qPCR

BGNC-F (0.4); BAB722 (0.4)

B. vogeli sp-sp cox1 qPCR

BCV-cox1-F4 (0.4); BCV-cox1-R (0.4)

B. canis sp-sp cox1 qPCR

BCC-cox1-F2 (0.4); BCC-cox1-R (0.4)

B. coco sp-sp cox1 qPCR

BCO-cox1-F2 (0.4); BCO-cox1-R2 (0.4)

B. gibsoni sp-sp cox1 qPCR

BG-cox1-F (0.4); BG-cox1-R (0.4)