Table 4 Retrospective analysis qPCR results. Retrospective qPCRs were performed simultaneously on previously characterized diagnostic or research samples from mammals that were uninfected (n = 4), naturally infected with Babesia or Cytauxzoon species (n = 31) or other vector-borne pathogens (n = 13)
Characterized sample (source) | Babesia 18S qPCR | Babesia LSU qPCR | ||
|---|---|---|---|---|
|  | Cq | T m (°C) | Cq | T m (°C) |
B. bovis (cow) | 32.2 | 86.5 | 17.0 | 76.5 |
B. caballi (horse) | 29.4 | 86 | 29 | 77 |
B. caballi (horse) | 36.3 | 86 | 35.1 | 76.5 |
B. canis (dog) | 33.7 | 86 | 32.9 | 77 |
B. canis (dog) | 36.7 | 86 | 36.8 | 77 |
B. canis (dog) | 32.5 | 86 | 31.4 | 77 |
B. rossi (dog) | 23.7 | 86 | 22.8 | 76.5 |
B. vogeli (dog) | 21.9 | 86.5 | 20.5 | 77 |
B. vogeli (dog) | 21.1 | 86 | 17.8 | 76.5 |
B. coco (dog) | 34.7 | 86 | 27.8 | 77 |
B. coco (dog) | 32.1 | 86 | 25.1 | 77 |
B. coco (dog) | – | none | 40.6 | 77 |
B. conradae (dog) | 34.2 | 86 | 19.8 | 76 |
B. conradae (dog) | 39 | 86 | 19.7 | 76 |
B. conradae (dog) | – | none | 17.3 | 76 |
B. conradae (dog) | – | none | 21.4 | 76.5 |
B. gibsoni (dog) | 13.2 | 86 | 13.01 | 77 |
B. gibsoni (dog) | 19.3 | 86 | 19.2 | 77 |
B. lengau (leopard) | 38.2 | 85.5 | 18 | 76 |
B. microti-like (dog) | – | none | 30.8 | 77 |
B. microti-like (dog) | – | none | 40.3 | 76.5 |
B. microti-like (dog) | 32 | 86 | 24 | 76.5 |
B. microti-like (red fox) | 36.5 | 85.5 | 27.8 | 76 |
B. microti-like (grey fox) | 36.6 | 85.5 | 28.2 | 76 |
B. odocoilei (reindeer) | 16.5 | 86 | 23.9 | 77 |
B. odocoilei (reindeer) | 13.5 | 86.5 | 25.5 | 77 |
B. odocoilei (elk) | 24.9 | 86.5 | 35.6 | 76.5 |
B. (sensu stricto) (maned wolf) | 14.4 | 86 | 13.6 | 77 |
B. (sensu stricto) (bear) | 34.1 | 86 | 32.4 | 76.5 |
Cytauxzoon felis (cat) | 31.8 | 85.5 | 17.2 | 77.5 |
C. felis (cat) | 35.3 | 85.5 | 21.2 | 77.5 |
Uninfected gDNA (cat) | – | none | – | none |
Uninfected gDNA (cow) | – | none | – | none |
Uninfected gDNA (dog) | – | none | – | none |
Uninfected gDNA (horse) | – | none | – | none |
Anaplasma phagocytophilum (dog) | – | none | – | none |
A. platys (dog) | – | none | – | none |
Bartonella henselae (cat) | – | none | – | none |
Ehrlichia canis (dog) | – | none | – | none |
E. ewingii (dog) | – | none | – | none |
Hepatozoon canis (dog) | 39.0 | 85 | – | none |
H. americanum (dog) | – | none | – | none |
Leishmania infantum (dog) | – | none | – | none |
Mycoplasma hemocanis (dog) | – | none | – | none |
Neorickettsia risticii (culture) | – | none | – | none |
Rickettsia rickettsii (dog) | – | none | – | none |
Theileria equi (horse) | 38.9 | 86 | – | none |
Trypanosoma cruzi (dog) | – | none | – | none |