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Table 4 Retrospective analysis qPCR results. Retrospective qPCRs were performed simultaneously on previously characterized diagnostic or research samples from mammals that were uninfected (n = 4), naturally infected with Babesia or Cytauxzoon species (n = 31) or other vector-borne pathogens (n = 13)

From: Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Characterized sample (source) Babesia 18S qPCR Babesia LSU qPCR
  Cq T m (°C) Cq T m (°C)
B. bovis (cow) 32.2 86.5 17.0 76.5
B. caballi (horse) 29.4 86 29 77
B. caballi (horse) 36.3 86 35.1 76.5
B. canis (dog) 33.7 86 32.9 77
B. canis (dog) 36.7 86 36.8 77
B. canis (dog) 32.5 86 31.4 77
B. rossi (dog) 23.7 86 22.8 76.5
B. vogeli (dog) 21.9 86.5 20.5 77
B. vogeli (dog) 21.1 86 17.8 76.5
B. coco (dog) 34.7 86 27.8 77
B. coco (dog) 32.1 86 25.1 77
B. coco (dog) none 40.6 77
B. conradae (dog) 34.2 86 19.8 76
B. conradae (dog) 39 86 19.7 76
B. conradae (dog) none 17.3 76
B. conradae (dog) none 21.4 76.5
B. gibsoni (dog) 13.2 86 13.01 77
B. gibsoni (dog) 19.3 86 19.2 77
B. lengau (leopard) 38.2 85.5 18 76
B. microti-like (dog) none 30.8 77
B. microti-like (dog) none 40.3 76.5
B. microti-like (dog) 32 86 24 76.5
B. microti-like (red fox) 36.5 85.5 27.8 76
B. microti-like (grey fox) 36.6 85.5 28.2 76
B. odocoilei (reindeer) 16.5 86 23.9 77
B. odocoilei (reindeer) 13.5 86.5 25.5 77
B. odocoilei (elk) 24.9 86.5 35.6 76.5
B. (sensu stricto) (maned wolf) 14.4 86 13.6 77
B. (sensu stricto) (bear) 34.1 86 32.4 76.5
Cytauxzoon felis (cat) 31.8 85.5 17.2 77.5
C. felis (cat) 35.3 85.5 21.2 77.5
Uninfected gDNA (cat) none none
Uninfected gDNA (cow) none none
Uninfected gDNA (dog) none none
Uninfected gDNA (horse) none none
Anaplasma phagocytophilum (dog) none none
A. platys (dog) none none
Bartonella henselae (cat) none none
Ehrlichia canis (dog) none none
E. ewingii (dog) none none
Hepatozoon canis (dog) 39.0 85 none
H. americanum (dog) none none
Leishmania infantum (dog) none none
Mycoplasma hemocanis (dog) none none
Neorickettsia risticii (culture) none none
Rickettsia rickettsii (dog) none none
Theileria equi (horse) 38.9 86 none
Trypanosoma cruzi (dog) none none
  1. Abbreviations: C q quantification cycle, T m melting temperature; −, Babesia was not amplified