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Table 6 Prospective analysis was performed on canine diagnostic samples naturally infected with Babesia spp. and quantification cycles (Cq) were compared between the 19 positive samples detected by Babesia genus assays (18S qPCR or LSU qPCR) and species-specific assays (18S sp-sp or cox1 sp-sp). Results are shown from the original Babesia genus qPCRs, discordant samples that were repeated in triplicate with the Babesia genus qPCRs, and Babesia species-specific qPCRs. All positive samples generated the correct melting temperature (T m ) values (not shown)
| Genus level qPCR | Species-specific qPCR | |||||
|---|---|---|---|---|---|---|
| Original results | Repeated results (triplicate) | |||||
| Babesia spp. | 18S | LSU | 18S | LSU | 18S | cox1 |
| B. canis | 31.4 | 30.5 | na | na | 31.6 | 30.0 |
| B. canis | 31.7 | 29.9 | na | na | 31.8 | 31.3 |
| B. vogeli | 30.6 | 29.4 | na | na | 31.3 | 31.4 |
| B. vogeli | 32.1 | 32.1 | na | na | 31.1 | 31.7 |
| B. gibsoni | 13.8 | 13.9 | na | na | 14.8 | 16.3 |
| B. gibsoni | 17.7 | 19.6 | na | na | 19.5 | 22.9 |
| B. gibsoni | 19.6 | 18.0 | na | na | 21.8 | 24.4 |
| B. gibsoni | 21.6 | 22.2 | na | na | 30.1 | 23.0 |
| B. gibsoni | 29.2 | 30.4 | na | na | 29.2 | 30.8 |
| B. gibsoni | 18.0 | 18.0 | na | na | 19.3 | 19.7 |
| B. gibsoni | 13.5 | 13.3 | na | na | 14.5 | 15.8 |
| B. coco | – | 39.8 | – | 38.5; 38.6; 33.8 | – | 39.0 |
| B. coco | – | 38.2 | – | 39.2; 33.1; − | – | 36.0 |
| B. gibsoni | – | 37.5 | – | – | – | – |
| B. gibsoni | – | 39.4 | – | 40.7; 38.3; 38.4 | 39 | 37.0 |
| B. gibsoni | – | 30.9 | – | 29.4; 29.7; 30.6 | – | 31.1 |
| B. gibsoni | – | 39.0 | 37.0; −; − | 38.4; −; − | – | 36.2 |
| B. gibsoni | – | 38.4 | – | 39.5; 38.6; − | – | 39.0 |
| B. microti-like | – | 29.0 | – | 31.0; 30.6; 30.3 | 32 | na |
