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Table 6 Prospective analysis was performed on canine diagnostic samples naturally infected with Babesia spp. and quantification cycles (Cq) were compared between the 19 positive samples detected by Babesia genus assays (18S qPCR or LSU qPCR) and species-specific assays (18S sp-sp or cox1 sp-sp). Results are shown from the original Babesia genus qPCRs, discordant samples that were repeated in triplicate with the Babesia genus qPCRs, and Babesia species-specific qPCRs. All positive samples generated the correct melting temperature (T m ) values (not shown)

From: Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

  Genus level qPCR Species-specific qPCR
  Original results Repeated results (triplicate)   
Babesia spp. 18S LSU 18S LSU 18S cox1
B. canis 31.4 30.5 na na 31.6 30.0
B. canis 31.7 29.9 na na 31.8 31.3
B. vogeli 30.6 29.4 na na 31.3 31.4
B. vogeli 32.1 32.1 na na 31.1 31.7
B. gibsoni 13.8 13.9 na na 14.8 16.3
B. gibsoni 17.7 19.6 na na 19.5 22.9
B. gibsoni 19.6 18.0 na na 21.8 24.4
B. gibsoni 21.6 22.2 na na 30.1 23.0
B. gibsoni 29.2 30.4 na na 29.2 30.8
B. gibsoni 18.0 18.0 na na 19.3 19.7
B. gibsoni 13.5 13.3 na na 14.5 15.8
B. coco 39.8 38.5; 38.6; 33.8 39.0
B. coco 38.2 39.2; 33.1; − 36.0
B. gibsoni 37.5
B. gibsoni 39.4 40.7; 38.3; 38.4 39 37.0
B. gibsoni 30.9 29.4; 29.7; 30.6 31.1
B. gibsoni 39.0 37.0; −; − 38.4; −; − 36.2
B. gibsoni 38.4 39.5; 38.6; − 39.0
B. microti-like 29.0 31.0; 30.6; 30.3 32 na
  1. Abbreviations: na, the sample was not retested; −, Babesia was not amplified