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Table 7 Positive percent agreement (PPA) and negative percent agreement (NPA)

From: Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

  Positive Negative Row sum
LSU qPCR (index) 18S qPCR (non-reference standard)  
Positivea 11 8 19
Negativeb 0 375 375
Column sum 11 383 394
18S qPCR (index) LSU qPCR (non-reference standard)  
Positivec 11 0 11
Negatived 8 375 383
Column sum 19 375 394
  1. Note: We calculated PPA and NPA using 18S qPCR assay (top) or LSU qPCR assay as the non-reference standard
  2. aPPA = 100% (95% CI: 69.9–100%)
  3. bNPA = 98% (95% CI: 95.9–99.0%)
  4. cPPA = 58% (95% CI: 36.2–76.9%)
  5. dNPA = 100% (95% CI: 98.8–100%)