Improved molecular detection of Babesia infections in animals using a novel quantitative real-time PCR diagnostic assay targeting mitochondrial DNA

Table 7 Positive percent agreement (PPA) and negative percent agreement (NPA)

	Positive	Negative	Row sum
LSU qPCR (index)	18S qPCR (non-reference standard)
Positive^a	11	8	19
Negative^b	0	375	375
Column sum	11	383	394
18S qPCR (index)	LSU qPCR (non-reference standard)
Positive^c	11	0	11
Negative^d	8	375	383
Column sum	19	375	394

Note: We calculated PPA and NPA using 18S qPCR assay (top) or LSU qPCR assay as the non-reference standard
^aPPA = 100% (95% CI: 69.9–100%)
^bNPA = 98% (95% CI: 95.9–99.0%)
^cPPA = 58% (95% CI: 36.2–76.9%)
^dNPA = 100% (95% CI: 98.8–100%)

ISSN: 1756-3305