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Table 1 Screening of bird blood samples for haemosporidian infections. The first column shows the number of positive samples out of the total screened (203 in Indiaa, 191 in Swedenb) for each of the methods. The columns to the right (neg) show the number of samples that were negative for the other methods

From: Estimating prevalence of avian haemosporidians in natural populations: a comparative study on screening protocols

 

Positive (Prevalence %)

Nestedneg

Enzneg

qPCRneg

Smearneg

India

 Nested

134 (66.0)

na

7

1

7

 Enz

188 (92.6)

53

na

3

10

 qPCR

199 (99.0)

63

20

na

13

 Smear

187 (92.1)

61

17

3

na

 Total

199 (99.0)

    

Sweden

 Nested

103 (53.9)

na

10

7

33

 Enz

95 (49.7)

2

na

3

25

 qPCR

103 (53.9)

7

11

na

33

 Smear

71 (37.17)

1

1

1

na

 Total

111 (58.1)

    
  1. Abbreviations: Nested nested protocol, ENZ restriction enzyme-based assay, qPCR quantitative PCR, Smear microscopy slides, na not applicable
  2. aSamples with known parasite intensities based on microscopy, including 16 smear negative samples
  3. bSamples selected irrespective of results by microscopy