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Table 1 Screening of bird blood samples for haemosporidian infections. The first column shows the number of positive samples out of the total screened (203 in Indiaa, 191 in Swedenb) for each of the methods. The columns to the right (neg) show the number of samples that were negative for the other methods

From: Estimating prevalence of avian haemosporidians in natural populations: a comparative study on screening protocols

  Positive (Prevalence %) Nestedneg Enzneg qPCRneg Smearneg
India
 Nested 134 (66.0) na 7 1 7
 Enz 188 (92.6) 53 na 3 10
 qPCR 199 (99.0) 63 20 na 13
 Smear 187 (92.1) 61 17 3 na
 Total 199 (99.0)     
Sweden
 Nested 103 (53.9) na 10 7 33
 Enz 95 (49.7) 2 na 3 25
 qPCR 103 (53.9) 7 11 na 33
 Smear 71 (37.17) 1 1 1 na
 Total 111 (58.1)     
  1. Abbreviations: Nested nested protocol, ENZ restriction enzyme-based assay, qPCR quantitative PCR, Smear microscopy slides, na not applicable
  2. aSamples with known parasite intensities based on microscopy, including 16 smear negative samples
  3. bSamples selected irrespective of results by microscopy