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Fig. 5 | Parasites & Vectors

Fig. 5

From: Secreted phospholipase A2 of Clonorchis sinensis activates hepatic stellate cells through a pathway involving JNK signalling

Fig. 5

Construction of mutant of MBP-CssPLA2 without enzymatic activity. a PCR product of the plasmid of the mutant. Lane M: DNA molecular marker; Lane 1: ddH2O negative control; Lane 2: PCR product of mutant of MBP-CssPLA2. b Detection of enzymatic activity of the mutant. c Sequence of the mutant plasmid of CssPLA2/pMAL-c2X. d Sequence of the plasmid of CssPLA2/pMAL-c2X

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