Fig. 9

JNK signalling is activated in HSCs after incubated with either MBP-CssPLA2 or the mutant. The marker of the activation, P-JNK1 of the hepatic stellate cells, was measured after incubated with 25 μg ml MBP, 25 μg/ml MBP-CssPLA2, 25 μg/ml mutant and PBS respectively. a Lane 1: HSCs incubated with 25 μg/ml MBP; Lane 2: HSCs incubated with 25 μg/ml MBP-CssPLA2; Lane 3: HSCs incubated with 25 μg/ml mutant; Lane 4: HSCs incubated with PBS. b Quantitative western blot analysis of P-JNK1. Compared with MBP, both MBP-CssPLA2 and the mutant could activate JNK signalling. There was no significant difference between MBP-CssPLA2 and the mutant in phosphorylation of JNK1. Unpaired t-test was applied for statistical analysis