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Fig. 10 | Parasites & Vectors

Fig. 10

From: Ultrastructure and localization of Neorickettsia in adult digenean trematodes provides novel insights into helminth-endobacteria interaction

Fig. 10

Comparative immunohistological detection of endobacteria using the APAAP method (a, b, d, f) and immunofluorescence (c, e, g). a Section of the ventral sucker stained with a pre-immune serum showing no red labeling for endobacteria. b Another section of a sucker showing red labeled endobacteria (arrows) in the sucker and the surrounding tegumental syncytium and parenchyma. c Consecutive section to a showing distinct labeling of endobacteria (arrows) in the sucker and parenchyma with high resolution by immunofluorescence. d, e Endobacteria (arrows) in the tegument and vitelline follicles. Note the more sensitive detection in e. f, g Endobacteria (arrows) in the outer epithelium of the trematode gut. Abbreviations: g, gut; os, oral sucker; p, parenchyma; ts, tegumental syncytium; vf, vitelline follicle; vs, ventral sucker. Scale-bars: a-c, 100 μm; d, e, 25 μm; f, g, 10 μm

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