Skip to main content

Table 2 Characteristics of candidate antigens from the R. microplus transcriptome selected using reverse vaccinology for assessment in an anti-tick vaccine

From: Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations

Transcripts from cDNA libraries (RMallHxN dataset) Recombinant protein Full-length sequencee
  Developmental stage No. ESTs in RmRa Expected No. ESTs in RmRj No. ESTs in RmSb Expected No. ESTs in RmS j P valuec   Amino acids MWd (kDa)   Amino acids MW (kDa)
Rm39 All stages 21 30 44 34 0.033f Rm39 54 5.8 Rm39 105 11
Rm180 Female, Nymph, Male 5 14 27 18 < 0.001g Rm180 67 7.9 Rm180 186 20.8
Rm239 Nymph, Male 7 18 26 14 0.007h Rm239 145 16.5 Rm239 500 55
  1. a R. microplus ticks fed on resistant hosts
  2. b R. microplus ticks fed on susceptible hosts
  3. cChi-square test using SigmaPlot 11.0 software
  4. dMolecular weight calculated without a histidine tag. The size of tagged recombinant proteins is shown in Additional file 2: Figure S1
  5. eComplete sequences of antigens
  6. fChi-square test, χ 2 = 4.531, df = 1
  7. gChi-square test, χ 2 = 10.864, df = 1
  8. hChi-square test, χ 2 = 7.343, df = 1
  9. jExpected counts were obtained from software report