Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations

Table 2 Characteristics of candidate antigens from the R. microplus transcriptome selected using reverse vaccinology for assessment in an anti-tick vaccine

Transcripts from cDNA libraries (RMallHxN dataset)							Recombinant protein			Full-length sequence^e
	Developmental stage	No. ESTs in RmR^a	Expected No. ESTs in RmR^j	No. ESTs in RmS^b	Expected No. ESTs in RmS ^j	P value^c		Amino acids	MW^d (kDa)		Amino acids	MW (kDa)
Rm39	All stages	21	30	44	34	0.033^f	Rm39	54	5.8	Rm39	105	11
Rm180	Female, Nymph, Male	5	14	27	18	< 0.001^g	Rm180	67	7.9	Rm180	186	20.8
Rm239	Nymph, Male	7	18	26	14	0.007^h	Rm239	145	16.5	Rm239	500	55

^a R. microplus ticks fed on resistant hosts
^b R. microplus ticks fed on susceptible hosts
^cChi-square test using SigmaPlot 11.0 software
^dMolecular weight calculated without a histidine tag. The size of tagged recombinant proteins is shown in Additional file 2: Figure S1
^eComplete sequences of antigens
^fChi-square test, χ ² = 4.531, df = 1
^gChi-square test, χ ² = 10.864, df = 1
^hChi-square test, χ ² = 7.343, df = 1
^jExpected counts were obtained from software report

ISSN: 1756-3305