Fig. 5

Comparative in vitro culture growth curves of B. bovis T3Bo, transfected 6-Cys EKO, and 6-Cys EKO-cln parasite lines with and without addition of inhibitory doses of blasticidin. The data are expressed as arithmetic means ± standard deviation (SD). P < 0.05 indicates a statistically significant difference and P > 0.05 indicating a statistically no significant difference. a In vitro culture growth curves of T3Bo strain without (blue line) and with the addition of blasticidin (red line). (t ₍₁₂₎ = 4.1, P = 0.0013). b In vitro culture growth curves of the 6-Cys EKO without (blue line), and with blasticidin addition (red line) (t ₍₁₂₎ = -0.55, P = 0.58). c In vitro culture growth curves of 6-Cys EKO-cln line without (blue line) or with blasticidin addition (red line) (t ₍₁₂₎ = 0.87, P = 0.399). d In vitro culture growth curves of T3Bo (blue line), 6-Cys EKO strain (red line), and 6-Cys EKO-cln line (green line) with blasticidin addition. Statistical comparisons: T3Bo vs 6-Cys EKO lines (t ₍₁₂₎ = 3.1, P = 0.009); T3Bo vs 6-Cys EKO-cln line (t ₍₁₂₎ = 3.6, P = 0.003). e In vitro culture growth curves of T3Bo strain (blue line), 6-Cys EKO (red line) and 6-Cys EKO-cln lines (green line) without blasticidin addition. Statistical comparisons: T3Bo vs 6-Cys EKO lines (t ₍₁₂₎ = -1.6, P = 0.13); T3Bo vs 6-Cys EKO-cln line (t ₍₁₂₎ = -0.25, P = 0.80)