Table 1 Primers sequence used in PCR and sequencing
From: Geno- and phenotypic characteristics of a transfected Babesia bovis 6-Cys-E knockout clonal line
Amplicon # | Primer pair |
|---|---|
1 | For: 5′-gctaagcacctatttagcgtaac-3′ Rev: 5′-gaccataagcttagtaaacgatagaacagactaag-3′ |
2 | For: 5′-atgaagcgaaatatcgtacacaatacc-3′ Rev:5′-atcgcaaagctttttcgtaaagttgcaataaattatc-3′ |
3 | For: 5′-ctgatcaagctttatatctgagacaacattagtatcg-3′ Rev: 5′-ctacgaggatcctccttt gtgaggttcacg-3′ |
4 | For: 5′-gctactgaattcatggtgagcaagggcgag-3′ Rev: 5′-ctacgaggatcctccttt gtgaggttcacg-3′ |
5 | For: 5′-gctactgaattcatggtgagcaagggcgag-3′ Rev: 5′-atgtcaatagtgatcatcg-3′ |
6 | For: 5-gctactctgcaggatgagatgcgtttataatg-3′ Rev: 5′-gttgcaaaaacatgttatgaag-3′ |
7 | For: 5′-ttgatggtaatatgaggc-3′ Rev: 5′-gttgcaaaaacatgttatgaag-3′ |