Fig. 2

Frequencies of CD8⁺; CD4^+; DP; DN lymphocytes; NK and NKT cells expressing CD107a from lesions of cutaneous leishmaniasis patients. Flow cytometry-representative analysis: a CD107a⁺DN T; b CD107a⁺NKT; c CD107a⁺CD4⁺ T; d CD107a⁺NK; e CD107a⁺DP T; f CD107a⁺CD8⁺ T cells. Isotype control (g) and fluorescence minus one (FMO) for CD107a staining (h). Electronic gates were created surrounding NKT, NK, CD4, DP, CD8 and DN cell populations (see Fig. 1). i The bars graph representing the mean ± SEM of percentages of NKT and NK cells; CD4⁺, DP, CD8⁺ and DN T lymphocytes expressing CD107a obtained from cutaneous leishmaniasis lesions of 18 patients. Statistical analyses between two groups were performed using Mann Whitney non-parametric t-test. Results were considered significant with P < 0.05 (*P < 0.05; **P < 0.01; ***P < 0.001)