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Fig. 2 | Parasites & Vectors

Fig. 2

From: CD3+CD4negCD8neg (double negative) T lymphocytes and NKT cells as the main cytotoxic-related-CD107a+ cells in lesions of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis

Fig. 2

Frequencies of CD8+; CD4+; DP; DN lymphocytes; NK and NKT cells expressing CD107a from lesions of cutaneous leishmaniasis patients. Flow cytometry-representative analysis: a CD107a+DN T; b CD107a+NKT; c CD107a+CD4+ T; d CD107a+NK; e CD107a+DP T; f CD107a+CD8+ T cells. Isotype control (g) and fluorescence minus one (FMO) for CD107a staining (h). Electronic gates were created surrounding NKT, NK, CD4, DP, CD8 and DN cell populations (see Fig. 1). i The bars graph representing the mean ± SEM of percentages of NKT and NK cells; CD4+, DP, CD8+ and DN T lymphocytes expressing CD107a obtained from cutaneous leishmaniasis lesions of 18 patients. Statistical analyses between two groups were performed using Mann Whitney non-parametric t-test. Results were considered significant with P < 0.05 (*P < 0.05; **P < 0.01; ***P < 0.001)

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