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Table 2 Agreement (%) between the results of multiplexed-tandem (MT)-PCR eggs versus larval culture (LC) and MT-PCR larvae (larvae) versus LC

From: Multiplexed-tandem PCR for the specific diagnosis of gastrointestinal nematode infections in sheep: an European validation study

Species n +,+ +,- -,+ -,- Agreement (%) Kappa PABAK
MT-PCR   Larval culture - Moredun (22 °C)
Te. circumcincta (MT-PCR (e)) 11 9 1 1 0 81.80 -0.100 0.636
Te. circumcincta (MT-PCR(l)) 11 7 0 3 1 72.70 0.298 0.455
Haemonchus (MT-PCR (e)) 11 1 0 0 10 100.00 1.000 1.000
Haemonchus (MT-PCR(l)) 11 1 0 0 10 100.00 1.000 1.000
Trichostrongylus (MT-PCR (e)) 11 6 2 0 3 81.80 0.620 0.636
Trichostrongylus (MT-PCR(l)) 11 6 4 0 1 63.60 0.214 0.273
Chabertia/Oesophagostomum (MT-PCR (e)) 11 8 0 1 2 90.90 0.744 0.818
Chabertia/Oesophagostomum (MT-PCR(l)) 11 8 0 1 2 90.90 0.744 0.818
C. curticei (MT-PCR (e)) 11 1 0 1 9 90.90 0.621 0.818
C. curticei (MT-PCR(l)) 11 1 0 1 9 90.90 0.621 0.818
   Larval culture - Belgium (25 °C)
Te. circumcincta (MT-PCR (e)) 8 8 0 0 0 100.00 -1.000 1.000
Te. circumcincta (MT-PCR(l)) 8 8 0 0 0 100.00 -1.000 1.000
Haemonchus (MT-PCR (e)) 8 6 1 0 1 87.50 0.600 0.750
Haemonchus (MT-PCR(l)) 8 5 1 1 1 75.00 0.330 0.500
Trichostrongylus (MT-PCR (e)) 8 3 2 2 1 50.00 -0.067 0.000
Trichostrongylus (MT-PCR(l)) 8 2 3 3 0 25.00 -0.600 -0.500
Chabertia/Oesophagostomum (MT-PCR (e)) 8 3 2 0 3 75.00 0.529 0.500
Chabertia/Oesophagostomum (MT-PCR(l)) 8 3 0 0 5 100.00 1.000 1.000
C. curticei (MT-PCR (e)) 8 2 1 0 5 87.50 0.714 0.750
C. curticei (MT-PCR(l)) 8 1 3 1 3 50.00 0.000 0.000
   Larval culture - Whole dataset (22 °C and 25 °C)
Te. circumcincta (MT-PCR (e)) 19 17 1 1 0 89.50 -0.056 0.789
Te. circumcincta (MT-PCR(l)) 19 15 0 3 1 84.20 0.345 0.684
Haemonchus (MT-PCR (e)) 19 7 1 0 11 94.70 0.890 0.895
Haemonchus (MT-PCR(l)) 19 6 1 1 11 89.50 0.774 0.789
Trichostrongylus (MT-PCR (e)) 19 9 4 2 4 68.40 0.329 0.368
Trichostrongylus (MT-PCR(l)) 19 8 7 3 1 47.40 -0.159 -0.053
Chabertia/Oesophagostomum (MT-PCR (e)) 19 11 2 1 5 84.20 0.650 0.684
Chabertia/Oesophagostomum (MT-PCR(l)) 19 11 0 1 7 94.70 0.890 0.895
C. curticei (MT-PCR (e)) 19 3 1 1 14 89.50 0.683 0.789
C. curticei (MT-PCR(l)) 19 2 3 2 12 73.70 0.275 0.474
  1. Also shown are the calculated Kappa values and the numbers of samples tested positive and negative by each method and for each laboratory as well as for the entire dataset