Table 2 Agreement (%) between the results of multiplexed-tandem (MT)-PCR eggs versus larval culture (LC) and MT-PCR larvae (larvae) versus LC
Species | n | +,+ | +,- | -,+ | -,- | Agreement (%) | Kappa | PABAK |
|---|---|---|---|---|---|---|---|---|
MT-PCR |  | Larval culture - Moredun (22 °C) | ||||||
  Te. circumcincta (MT-PCR (e)) | 11 | 9 | 1 | 1 | 0 | 81.80 | -0.100 | 0.636 |
  Te. circumcincta (MT-PCR(l)) | 11 | 7 | 0 | 3 | 1 | 72.70 | 0.298 | 0.455 |
  Haemonchus (MT-PCR (e)) | 11 | 1 | 0 | 0 | 10 | 100.00 | 1.000 | 1.000 |
  Haemonchus (MT-PCR(l)) | 11 | 1 | 0 | 0 | 10 | 100.00 | 1.000 | 1.000 |
  Trichostrongylus (MT-PCR (e)) | 11 | 6 | 2 | 0 | 3 | 81.80 | 0.620 | 0.636 |
  Trichostrongylus (MT-PCR(l)) | 11 | 6 | 4 | 0 | 1 | 63.60 | 0.214 | 0.273 |
  Chabertia/Oesophagostomum (MT-PCR (e)) | 11 | 8 | 0 | 1 | 2 | 90.90 | 0.744 | 0.818 |
  Chabertia/Oesophagostomum (MT-PCR(l)) | 11 | 8 | 0 | 1 | 2 | 90.90 | 0.744 | 0.818 |
  C. curticei (MT-PCR (e)) | 11 | 1 | 0 | 1 | 9 | 90.90 | 0.621 | 0.818 |
  C. curticei (MT-PCR(l)) | 11 | 1 | 0 | 1 | 9 | 90.90 | 0.621 | 0.818 |
|  |  | Larval culture - Belgium (25 °C) | ||||||
  Te. circumcincta (MT-PCR (e)) | 8 | 8 | 0 | 0 | 0 | 100.00 | -1.000 | 1.000 |
  Te. circumcincta (MT-PCR(l)) | 8 | 8 | 0 | 0 | 0 | 100.00 | -1.000 | 1.000 |
  Haemonchus (MT-PCR (e)) | 8 | 6 | 1 | 0 | 1 | 87.50 | 0.600 | 0.750 |
  Haemonchus (MT-PCR(l)) | 8 | 5 | 1 | 1 | 1 | 75.00 | 0.330 | 0.500 |
  Trichostrongylus (MT-PCR (e)) | 8 | 3 | 2 | 2 | 1 | 50.00 | -0.067 | 0.000 |
  Trichostrongylus (MT-PCR(l)) | 8 | 2 | 3 | 3 | 0 | 25.00 | -0.600 | -0.500 |
  Chabertia/Oesophagostomum (MT-PCR (e)) | 8 | 3 | 2 | 0 | 3 | 75.00 | 0.529 | 0.500 |
  Chabertia/Oesophagostomum (MT-PCR(l)) | 8 | 3 | 0 | 0 | 5 | 100.00 | 1.000 | 1.000 |
  C. curticei (MT-PCR (e)) | 8 | 2 | 1 | 0 | 5 | 87.50 | 0.714 | 0.750 |
  C. curticei (MT-PCR(l)) | 8 | 1 | 3 | 1 | 3 | 50.00 | 0.000 | 0.000 |
|  |  | Larval culture - Whole dataset (22 °C and 25 °C) | ||||||
  Te. circumcincta (MT-PCR (e)) | 19 | 17 | 1 | 1 | 0 | 89.50 | -0.056 | 0.789 |
  Te. circumcincta (MT-PCR(l)) | 19 | 15 | 0 | 3 | 1 | 84.20 | 0.345 | 0.684 |
  Haemonchus (MT-PCR (e)) | 19 | 7 | 1 | 0 | 11 | 94.70 | 0.890 | 0.895 |
  Haemonchus (MT-PCR(l)) | 19 | 6 | 1 | 1 | 11 | 89.50 | 0.774 | 0.789 |
  Trichostrongylus (MT-PCR (e)) | 19 | 9 | 4 | 2 | 4 | 68.40 | 0.329 | 0.368 |
  Trichostrongylus (MT-PCR(l)) | 19 | 8 | 7 | 3 | 1 | 47.40 | -0.159 | -0.053 |
  Chabertia/Oesophagostomum (MT-PCR (e)) | 19 | 11 | 2 | 1 | 5 | 84.20 | 0.650 | 0.684 |
  Chabertia/Oesophagostomum (MT-PCR(l)) | 19 | 11 | 0 | 1 | 7 | 94.70 | 0.890 | 0.895 |
  C. curticei (MT-PCR (e)) | 19 | 3 | 1 | 1 | 14 | 89.50 | 0.683 | 0.789 |
  C. curticei (MT-PCR(l)) | 19 | 2 | 3 | 2 | 12 | 73.70 | 0.275 | 0.474 |