Fig. 4
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13071-017-2181-x/MediaObjects/13071_2017_2181_Fig4_HTML.gif)
qPCR-ama standard curves constructed with serial dilutions of L. (L.) amazonensis MHOM/BR/00/LTB0016 DNA. The standard curves were obtained with serial dilutions ranging from 1.0 to 0.0001 ng/tube, spiked (upper curve, y = -3.3720x + 24.4850; R 2 = 0.9915) or non-spiked (lower curve, y =-3.3933x + 17.9160; R 2 = 0.9803) with 30 ng human DNA. Each point derived from a duplicate of two independent experiments