Fig. 7

Sustained effects of a 30 min light pulse on An. gambiae mosquito flight activity. Mean flight activity measured during a 60 min period at various intervals over 8 h (1, 2, 3, 4 and 8 h), following exposure to a 30 min light pulse. Analysis was conducted at times of the circadian cycle that correspond with changes in activity that were detected during the 30 min exposure to light, i.e. at ZT12 (a), ZT16 (b), ZT22 (c) and CT24/0 (d) (see also Fig. 6 for mean flight activity measured during the light pulse administration). Mean flight activity is compared on the day of treatment (triangle symbols) to activity during the same period on the two prior, non-pulsed days (circle and square symbols). A pulse administered at ZT22 significantly inhibited activity during 1 h following the pulse (i.e. at ZT22.5–23.5; Table 1). No other significant differences between treatment and control days were observed during the 8 h following the time-specific light pulses Table 1). One-way RM-ANOVAs followed by post-hoc tests (***P < 0.001). Values shown are mean ± S.E.M. of individual mosquito activity. Activity is scored as the average number of LAM beam crossings per minute during the 60 min duration determined for each individual mosquito at the specified time intervals