Fig. 1

Inflammasome-related gene expression in mouse peritoneal macrophages in response to N. caninum infection. The 96-well RT Profiler Mouse Inflammasome PCR Arrays (Qiagen) were probed with cDNA from peritoneal macrophages infected with N. caninum (MOI = 3:1, parasite:cell) for 12 h or 24 h (without LPS priming). The Ct values from the qPCR data were analyzed, and the relative expression level of each target gene in the samples was calculated using fold change. Fold-Change (2^-ΔΔCt) is the normalized gene expression (2^-ΔCt) in the Test Sample divided the normalized gene expression (2^-ΔCt) in the Control Sample. In the PCR arrays, 84 inflammasome-related genes were measured, and the expression levels of key genes in three functional groups are shown. a Relative expression levels of 16 NOD-like receptor genes. b Relative expression levels of downstream signaling genes in inflammasome-mediated responses. c Relative expression levels of inflammasome negative regulation genes. The data are representative of two independent experiments