Table 1 Primer sequences used in this study
From: NLRP3 inflammasome activation in murine macrophages caused by Neospora caninum infection
Gene | Accession number | Primer sequence (5'-3') | Size (bp) | Reference |
|---|---|---|---|---|
Murine | ||||
NLRP3 | NM_145827 | Forward: AGAAGAGACCACGGCAGAAG | 102 | [23] |
Reverse: CCTTGGACCAGGTTCAGTGT | ||||
NLRC4 | NM_001033367.3 | Forward: CTTGGCCAGGAGAGCCTTG | 153 | |
Reverse: GGGCTCGTCTGTTGTTCCTT | ||||
NLRC5 | NM_001033207.3 | Forward: GCTGAGAGCATCCGACTGAA | 157 | |
Reverse: GGTGGATGACCTCAGGGTTG | ||||
ACTB | NM_007393.5 | Forward: ACCTTCTACAATGAGCTGCG | 147 | |
Reverse: CTGGATGGCTACGTACATGG | ||||
Neospora caninum | ||||
NC5 | X84238 | Forward: ACTGGAGGCACGCTGAACAC | 76 | [25] |
Reverse: AACAATGCTTCGCAAGAGGAA | ||||