Fig. 6
Simultaneous alignments of nucleotide and amino-acid sequences of mitochondrial cox1 genes from Ancylostoma ailuropodae n. sp. and its congeneric species. For the alignments, the nucleotide sequences of cox1 genes were retrieved from the GenBank database (species and accession numbers are indicated in parentheses): Aai (A. ailuropodae n. sp.; KP842921), Ace (A. ceylanicum; KF896601), Aca (A. caninum; AB751617), Adu (A. duodenale; NC_003415), and Atu (A. tubaeforme; AJ407940). The corresponding protein sequences were deduced based on the Invertebrate Mitochondrial Code. Both nucleotide and amino-acid sequences were aligned with Clustal X 1.83 program. Regions of identity in either nucleotide (*) or amino-acid (#) are indicated. Variable base loci in Aai unique for A. ailuropodae n. sp. are highlighted in red; those shared between A. ailuropodae n. sp. and one of A. ceylanicum, A. duodenale, A. caninum and A. tubaeforme are highlighted in orange; and those shared between A. ailuropodae n. sp. and any two or three of these four congeneric species are highlighted in yellow. The non-synonymous substitutions A/G250 in A. ceylanicum and T/A251 in A. caninum as well as their amino-acid changes: I (Ilu)/V (Val) and I (Ilu)/N (Asn) are noted in red with a red star. Percentages of nucleotide and amino-acid identities with respect to Aai are shown at the end of each sequence