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Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterization of Schistosoma japonicum tetraspanning orphan receptor and its role in binding to complement C2 and immunoprotection against murine schistosomiasis

Fig. 3

Analysis of expression and antigenicity of rSjTOR-ed1. a Expression and purification of SjTOR-ed1 in E. coli. Cell extracts and fractions from E. coli BL21 (DE3) transformed with pET28-SjTOR-ed1 were separated by 12% SDS-PAGE. Lanes A and B: total extract of a clone before and after induction with one mM IPTG; Lanes C and D: supernatant and inclusion bodies, respectively, after lysis; Lane E: rSjTOR-ed1 protein purified from inclusion bodies through Ni-NTA His-binding resin. b Western blot analysis of the antigenicity of rSjTOR-ed1. Lane M: molecular mass markers; Lanes A, B and C: purified rSjTOR-ed1 was probed with anti-rSjTOR-ed1 mouse serum (positive control), serum from mice infected with S. japonicum (experiment group), and normal mouse serum (negative control), respectively

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