Fig. 1

Allergic reactions in Anisakis pegreffii and Pseudoterranova decipiens sensitized mice. a Symptoms score in BALB/c mice. b Histamine release in BALB/c mouse sera. Green symbols and histograms: mice orally infected with A. pegreffii (L3 p.o.); red symbols and histograms: mice intraperitoneally infected with A. pegreffii or P. decipiens (L3 p.i.); blue symbols and histograms: mice orally immunized with A. pegreffii or P. decipiens crude worm extracts (CWE); open circles and histogram: mice (naïve) intraperitoneally inoculated with phosphate buffered saline (PBS). Symbols represent individual mice (combined results of 2–3 experiments). Horizontal bars represent medians. Reactions were scored following the protocol of Nieuwenhuizen et al. [14]. Histograms represent mean values with SE. Significances (*P < 0.05) were calculated between experimental and naïve control groups. Each experiment was conducted in triplicate