A review of Sarcoptes scabiei: past, present and future

Parasites & Vectors

Table 4 Characteristics of published ELISAs using recombinant proteins as the target antigen to detect antibodies circulating in serum of scabietic hosts

Target antigen		Host species	Detecting antibodies		Sensitivity (%)	Specificity (%)	Reference
Mite variety	rProtein		Primary	Conjugate
cunniculi	tropomyosin	rabbit		HRP-Go × Rb IgG			[141]
cunniculi	SsTPx	rabbit		HRP-Go × Rb IgG	100	100	[142]
cunniculi	cofilin	rabbit		HRP-Go × Rb IgG	83	88	[143]
cunniculi	calmodulin	rabbit		HRP-Go × Rb IgG	88	23	[109]
hominis	rSar s 14.3	pig		HRP-Rb × Pig IgG	100	100	[132]
hominis	rSar s 14.3	human	BT-Mu × Hu IgE	Eu-SA	100	94	[144]
hominis	Ssλ20ΔB3	chamois		HRP-Protein G	100	97	[107]
hominis	Ssλ20ΔB3	deer		HRP-Protein G	100	97	[107]
hominis	Ssλ20ΔB3	deer		HRP-Protein G	75	97	[106]
hominis	Ssλ20ΔB3	ibex		HRP-Protein G	58	78	[128]
hominis	Ssλ20ΔB3	pig		HRP-? × Pig IgG	40	86	[145]
hominis	Ssλ20ΔB3	pig		HRP-? × Pig IgG	62	83	[145]
hominis	Ssλ20ΔB3	rabbit		HRP-Protein A		100	[108]
hominis	Ssλ20ΔB3	rabbit		HRP-Protein A	95	97	[146]
hominis	Ssλ20ΔB3	wolf		HRP-Protein A	75	88	[147]
hominis	rSsGST01	human		AP-? × Hu IgV			[148]
hominis	rSj26GST	human		AP-? × Hu IgV			[148]
suis	SsE2	human		HRP-Go × Hu?	25		[111]
suis	SsE3	human		HRP-Go × Hu?	85		[111]
suis	SsE5	human		HRP-Go × Hu?	60		[111]
suis	SsE7	human		HRP-Go × Hu?	25		[111]
suis	SsE8	human		HRP-Go × Hu?	15		[111]
suis	SsE9	human		HRP-Go × Hu?	0		[111]
suis	SsE2	pig		HRP-Go × Pig?	4.8		[111]
suis	SsE3	pig		HRP-Go × Pig?	4.8		[111]
suis	SsE5	pig		HRP-Go × Pig?	4.8		[111]
suis	SsE7	pig		HRP-Go × Pig?	61.9		[111]
suis	SsE8	pig		HRP-Go × Pig?	4.8		[111]
suis	SsE9	pig		HRP-Go × Pig?	14.3		[111]

Abbreviations: AP alkaline phosphatase, BT, biotinylated, Eu europium, Go goat, HRP horseradish peroxidase, Hu human, Mu mouse, Rb rabbit, SA streptavidin, V various isotypes, x = anti-;? = not reported

ISSN: 1756-3305