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Table 4 Characteristics of published ELISAs using recombinant proteins as the target antigen to detect antibodies circulating in serum of scabietic hosts

From: A review of Sarcoptes scabiei: past, present and future

Target antigen Host species Detecting antibodies Sensitivity (%) Specificity (%) Reference
Mite variety rProtein   Primary Conjugate    
cunniculi tropomyosin rabbit   HRP-Go × Rb IgG    [141]
cunniculi SsTPx rabbit   HRP-Go × Rb IgG 100 100 [142]
cunniculi cofilin rabbit   HRP-Go × Rb IgG 83 88 [143]
cunniculi calmodulin rabbit   HRP-Go × Rb IgG 88 23 [109]
hominis rSar s 14.3 pig   HRP-Rb × Pig IgG 100 100 [132]
hominis rSar s 14.3 human BT-Mu × Hu IgE Eu-SA 100 94 [144]
hominis Ssλ20ΔB3 chamois   HRP-Protein G 100 97 [107]
hominis Ssλ20ΔB3 deer   HRP-Protein G 100 97 [107]
hominis Ssλ20ΔB3 deer   HRP-Protein G 75 97 [106]
hominis Ssλ20ΔB3 ibex   HRP-Protein G 58 78 [128]
hominis Ssλ20ΔB3 pig   HRP-? × Pig IgG 40 86 [145]
hominis Ssλ20ΔB3 pig   HRP-? × Pig IgG 62 83 [145]
hominis Ssλ20ΔB3 rabbit   HRP-Protein A   100 [108]
hominis Ssλ20ΔB3 rabbit   HRP-Protein A 95 97 [146]
hominis Ssλ20ΔB3 wolf   HRP-Protein A 75 88 [147]
hominis rSsGST01 human   AP-? × Hu IgV    [148]
hominis rSj26GST human   AP-? × Hu IgV    [148]
suis SsE2 human   HRP-Go × Hu? 25   [111]
suis SsE3 human   HRP-Go × Hu? 85   [111]
suis SsE5 human   HRP-Go × Hu? 60   [111]
suis SsE7 human   HRP-Go × Hu? 25   [111]
suis SsE8 human   HRP-Go × Hu? 15   [111]
suis SsE9 human   HRP-Go × Hu? 0   [111]
suis SsE2 pig   HRP-Go × Pig? 4.8   [111]
suis SsE3 pig   HRP-Go × Pig? 4.8   [111]
suis SsE5 pig   HRP-Go × Pig? 4.8   [111]
suis SsE7 pig   HRP-Go × Pig? 61.9   [111]
suis SsE8 pig   HRP-Go × Pig? 4.8   [111]
suis SsE9 pig   HRP-Go × Pig? 14.3   [111]
  1. Abbreviations: AP alkaline phosphatase, BT, biotinylated, Eu europium, Go goat, HRP horseradish peroxidase, Hu human, Mu mouse, Rb rabbit, SA streptavidin, V various isotypes, x = anti-;? = not reported