Fig. 3

rHc-AK protein binding to goat PBMCs. Localisation was conducted by incubation of PBMC with rat anti-rHc-AK-O IgG or negative rat IgG (control). a, b Staining of the target protein (red) was utilised by the Cy3-conjugated secondary antibody. c, d Nuclei of corresponding cells were stained with DAPI (blue). e, f A merge overlaps of red and blue channels visualised by confocal microscopy. No red fluorescence was observed in control group. Scale-bars: 10 μm