Fig. 6

Effect of the 14-3-3 inhibitor on the cytoskeleton organisation of C6/36 HT cells. The intracellular distribution of 14-3-3 proteins (green), actin (red), and nuclei (blue) on C6/36 HT cells treated with 14-3-3 inhibitor, DMSO and medium were visualised by Immunofluorescence. In medium or DMSO treated cells, 14-3-3 proteins were present mainly in the cytoplasm and did not co-localized with F-actin in the cortex. In contrast, cells treated with 14-3-3 inhibitors underwent morphologic changes, e.g. elongation through reorganisation of F-actin and colocalizing with 14-3-3 proteins. Scale-bars: 5 μm