Fig. 8

DsiRNAs mediated silencing of 14-3-3ε and 14-3-3ζ expression on Aag-2 cells. Aag-2 cells were double transfected with DsiRNA targeting 14-3-3, and expression of 14-3-3Ɛ and 14-3-3ζ proteins was assessed by Western blot. a Optical microscopy images of Aag-2 cells treated: (aI) DsiRNA control (Mock), (aII) DsiRNANeg, (aIII) DsiRNA14-3-3ζ and (aIV) DsiRNA 14-3-3Ɛ. b 14-3-3 protein expression on Aag-2 cells treated with control (Mock) (Lane 1), DsiRNANeg (Lane 2), DsiRNA14-3-3Ɛ (Lane 3) and DsiRNA14-3-3ζ (Lane 4). c The expression of 14-3-3 proteins normalised against actin. DsiRNAs successfully knockdown the expression of both 14-3-3ε and 14-3-3ζ in Aag-2 cells. Scale-bars: 100 μm