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Fig. 4 | Parasites & Vectors

Fig. 4

From: A dual colour fluorescence in situ hybridization (FISH) assay for identifying the zoonotic malaria parasite Plasmodium knowlesi with a potential application for the specific diagnosis of knowlesi malaria in peripheral-level laboratories of Southeast Asia

Fig. 4

Fluorescence observed in the PK-FISH assay with the P. knowlesi-specific probe (green), the Plasmodium genus-specific probe (orange) and the Babesia genus-specific probe (green) in specificity control blood smears prepared from hamster blood infected with B. duncani. Two smears (Smear 1 and Smear 2) were made with the same B. duncani-infected hamster blood (ATCC PRA-302). Smear 1 was tested in the PK- FISH Assay and Smear 2 was tested in a Babesia genus-specific FISH assay. The results viewed at ×1000 magnification show a absence of a reaction in the PK-FISH assay with the P. knowlesi-specific probe on Smear 1, b absence of a reaction in the PK-FISH assay in the same microscope field with the Plasmodium genus-specific probe on Smear 1, and c reaction with the Babesia genus-specific FISH probe on Smear 2. Scale-bars: 5 μm

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Parasites & Vectors

ISSN: 1756-3305

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