Table 1 List of identified differential metabolites involved in the perturbed metabolic pathways during acute and chronic phases of Toxoplasma gondii infection
From: Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis
Mice group | Ionization method | m/z - RT | Metabolites | VIP | FC | q-value | Metabolic pathways |
|---|---|---|---|---|---|---|---|
AI vs Con | ESI (+) | 369.205–5.980 | Corticosterone | 2.843 | 3.049 | 0.017 | Steroid hormone biosynthesis; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion |
433.342–7.128 | 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol | 5.216 | 9.497 | 0.040 | Steroid biosynthesis | ||
299.197–7.263 | Stearidonic acid | 1.819 | 0.732 | 0.025 | alpha-Linolenic acid metabolism | ||
453.293–7.612 | 7alpha-Hydroxy-3-oxo-4-cholestenoate | 1.462 | 0.704 | 0.026 | Primary bile acid biosynthesis | ||
454.390–7.684 | 3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestane | 1.369 | 0.331 | 0.011 | Primary bile acid biosynthesis | ||
331.224–7.727 | 17alpha,21-Dihydroxypregnenolone | 1.907 | 0.550 | 0.035 | Steroid hormone biosynthesis | ||
709.559–8.548 | Ubiquinone-8 | 4.574 | 0.0565 | 0.040 | Ubiquinone and other terpenoid-quinone biosynthesis | ||
ESI (−) | 303.232–7.199 | Arachidonic acid | 1.271 | 0.652 | 0.045 | Arachidonic acid metabolism; Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids; Vascular smooth muscle contraction; Platelet activation; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Retrograde endocannabinoid signaling; Serotonergic synapse; Long-term depression; Inflammatory mediator regulation of TRP channels; GnRH signaling pathway; Ovarian steroidogenesis; Oxytocin signaling pathway; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion | |
453.161–8.588 | Methotrexate | 1.537 | 0.731 | 0.047 | Bile secretion | ||
CI vs Con | ESI (+) | 433.342–7.128 | 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol; | 4.660 | 6.459 | 0.046 | Steroid biosynthesis |
454.390–7.684 | 3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestane | 1.185 | 0.385 | 0.014 | Primary bile acid biosynthesis | ||
385.344–7.886 | Cerebrosterol | 2.976 | 8.236 | 0.046 | Primary bile acid biosynthesis | ||
361.150–8.322 | Neamine | 3.163 | 4.633 | 0.023 | Butirosin and neomycin biosynthesis; Biosynthesis of antibiotics | ||
305.214–8.519 | 19-Hydroxytestosterone | 1.896 | 1.669 | 0.049 | Steroid hormone biosynthesis | ||
351.229–8.533 | Docosahexaenoic acid(DHA) | 1.113 | 1.331 | 0.033 | Biosynthesis of unsaturated fatty acids | ||
709.559–8.548 | Ubiquinone-8 | 7.038 | 0.019 | 0.002 | Ubiquinone and other terpenoid-quinone biosynthesis | ||
391.284–8.943 | Bile acid | 1.342 | 1.470 | 0.037 | Fat digestion and absorption; Vitamin digestion and absorption | ||
365.194–8.994 | Phorbol | 1.464 | 1.483 | 0.029 | Inflammatory mediator regulation of TRP channels | ||
419.350–9.386 | 7alpha,27-Dihydroxycholesterol | 1.509 | 2.112 | 0.032 | Primary bile acid biosynthesis | ||
425.339–9.728 | 7alpha-Hydroxycholesterol | 2.029 | 2.228 | 0.006 | Primary bile acid biosynthesis | ||
343.195–8.563 | Arachidonic acid | 2.132 | 1.757 | 0.015 | Arachidonic acid metabolism; Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids; Vascular smooth muscle contraction; Platelet activation; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Retrograde endocannabinoid signaling; Serotonergic synapse; Long-term depression; Inflammatory mediator regulation of TRP channels; GnRH signaling pathway; Ovarian steroidogenesis; Oxytocin signaling pathway; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion; | ||
ESI (−) | 295.227–10.292 | (9S)-Hydroxyoctadecadienoic acid | 1.707 | 0.702 | 0.020 | Linoleic acid metabolism; PPAR signaling pathway | |
317.210–7.655 | Leukotriene A4 | 1.364 | 1.623 | 0.020 | Arachidonic acid metabolism; Serotonergic synapse | ||
315.195–8.218 | 15-Deoxy-delta-12,14-PGJ2 | 1.834 | 1.942 | 0.005 | Arachidonic acid metabolism | ||
319.189–8.325 | Ubiquinol | 2.203 | 2.424 | 0.038 | Oxidative phosphorylation | ||
301.215–8.353 | (5Z,7E,9E,14Z,17Z)-Eicosapentaenoate | 3.001 | 3.042 | 0.017 | Inflammatory mediator regulation of TRP channels | ||
289.216–8.328 | Androstenediol | 2.718 | 3.058 | 0.048 | Steroid hormone biosynthesis; Ovarian steroidogenesis | ||
550.264–8.439 | Taurocholate | 1.654 | 1.936 | 0.023 | Primary bile acid biosynthesis; Taurine and hypotaurine metabolism; Bile secretion | ||
371.179–8.511 | Biocytin | 1.024 | 0.806 | 0.030 | Biotin metabolism; Vitamin digestion and absorption | ||
543.151–8.525 | Premithramycin A1 | 2.026 | 0.598 | 0.030 | Biosynthesis of antibiotics | ||
416.316–8.571 | N-Oleoyl dopamine | 2.167 | 3.359 | 0.024 | Neuroactive ligand-receptor interaction | ||
453.161–8.588 | Methotrexate | 1.686 | 0.701 | 0.008 | Bile secretion | ||
569.166–8.588 | 5″-Phosphoribostamycin | 1.823 | 0.538 | 0.045 | Butirosin and neomycin biosynthesis | ||
305.247–8.774 | (8Z,11Z,14Z)-Icosatrienoic acid | 1.630 | 1.519 | 0.031 | Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids | ||
307.262–9.009 | Icosadienoic acid | 1.767 | 1.819 | 0.006 | Biosynthesis of unsaturated fatty acids | ||
337.326–9.024 | 14,15-DHET | 1.702 | 1.557 | 0.029 | Arachidonic acid metabolism; Serotonergic synapse | ||
283.262–9.238 | Octadecanoic acid | 1.134 | 1.207 | 0.007 | Fatty acid biosynthesis; Biosynthesis of unsaturated fatty acids |