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Table 1 List of identified differential metabolites involved in the perturbed metabolic pathways during acute and chronic phases of Toxoplasma gondii infection

From: Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis

Mice group Ionization method m/z - RT Metabolites VIP FC q-value Metabolic pathways
AI vs Con ESI (+) 369.205–5.980 Corticosterone 2.843 3.049 0.017 Steroid hormone biosynthesis; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion
433.342–7.128 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol 5.216 9.497 0.040 Steroid biosynthesis
299.197–7.263 Stearidonic acid 1.819 0.732 0.025 alpha-Linolenic acid metabolism
453.293–7.612 7alpha-Hydroxy-3-oxo-4-cholestenoate 1.462 0.704 0.026 Primary bile acid biosynthesis
454.390–7.684 3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestane 1.369 0.331 0.011 Primary bile acid biosynthesis
331.224–7.727 17alpha,21-Dihydroxypregnenolone 1.907 0.550 0.035 Steroid hormone biosynthesis
709.559–8.548 Ubiquinone-8 4.574 0.0565 0.040 Ubiquinone and other terpenoid-quinone biosynthesis
ESI (−) 303.232–7.199 Arachidonic acid 1.271 0.652 0.045 Arachidonic acid metabolism; Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids; Vascular smooth muscle contraction; Platelet activation; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Retrograde endocannabinoid signaling; Serotonergic synapse; Long-term depression; Inflammatory mediator regulation of TRP channels; GnRH signaling pathway; Ovarian steroidogenesis; Oxytocin signaling pathway; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion
453.161–8.588 Methotrexate 1.537 0.731 0.047 Bile secretion
CI vs Con ESI (+) 433.342–7.128 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol; 4.660 6.459 0.046 Steroid biosynthesis
454.390–7.684 3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestane 1.185 0.385 0.014 Primary bile acid biosynthesis
385.344–7.886 Cerebrosterol 2.976 8.236 0.046 Primary bile acid biosynthesis
361.150–8.322 Neamine 3.163 4.633 0.023 Butirosin and neomycin biosynthesis; Biosynthesis of antibiotics
305.214–8.519 19-Hydroxytestosterone 1.896 1.669 0.049 Steroid hormone biosynthesis
351.229–8.533 Docosahexaenoic acid(DHA) 1.113 1.331 0.033 Biosynthesis of unsaturated fatty acids
709.559–8.548 Ubiquinone-8 7.038 0.019 0.002 Ubiquinone and other terpenoid-quinone biosynthesis
391.284–8.943 Bile acid 1.342 1.470 0.037 Fat digestion and absorption; Vitamin digestion and absorption
365.194–8.994 Phorbol 1.464 1.483 0.029 Inflammatory mediator regulation of TRP channels
419.350–9.386 7alpha,27-Dihydroxycholesterol 1.509 2.112 0.032 Primary bile acid biosynthesis
425.339–9.728 7alpha-Hydroxycholesterol 2.029 2.228 0.006 Primary bile acid biosynthesis
343.195–8.563 Arachidonic acid 2.132 1.757 0.015 Arachidonic acid metabolism; Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids; Vascular smooth muscle contraction; Platelet activation; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Retrograde endocannabinoid signaling; Serotonergic synapse; Long-term depression; Inflammatory mediator regulation of TRP channels; GnRH signaling pathway; Ovarian steroidogenesis; Oxytocin signaling pathway; Regulation of lipolysis in adipocytes; Aldosterone synthesis and secretion;
ESI (−) 295.227–10.292 (9S)-Hydroxyoctadecadienoic acid 1.707 0.702 0.020 Linoleic acid metabolism; PPAR signaling pathway
317.210–7.655 Leukotriene A4 1.364 1.623 0.020 Arachidonic acid metabolism; Serotonergic synapse
315.195–8.218 15-Deoxy-delta-12,14-PGJ2 1.834 1.942 0.005 Arachidonic acid metabolism
319.189–8.325 Ubiquinol 2.203 2.424 0.038 Oxidative phosphorylation
301.215–8.353 (5Z,7E,9E,14Z,17Z)-Eicosapentaenoate 3.001 3.042 0.017 Inflammatory mediator regulation of TRP channels
289.216–8.328 Androstenediol 2.718 3.058 0.048 Steroid hormone biosynthesis; Ovarian steroidogenesis
550.264–8.439 Taurocholate 1.654 1.936 0.023 Primary bile acid biosynthesis; Taurine and hypotaurine metabolism; Bile secretion
371.179–8.511 Biocytin 1.024 0.806 0.030 Biotin metabolism; Vitamin digestion and absorption
543.151–8.525 Premithramycin A1 2.026 0.598 0.030 Biosynthesis of antibiotics
416.316–8.571 N-Oleoyl dopamine 2.167 3.359 0.024 Neuroactive ligand-receptor interaction
453.161–8.588 Methotrexate 1.686 0.701 0.008 Bile secretion
569.166–8.588 5″-Phosphoribostamycin 1.823 0.538 0.045 Butirosin and neomycin biosynthesis
305.247–8.774 (8Z,11Z,14Z)-Icosatrienoic acid 1.630 1.519 0.031 Linoleic acid metabolism; Biosynthesis of unsaturated fatty acids
307.262–9.009 Icosadienoic acid 1.767 1.819 0.006 Biosynthesis of unsaturated fatty acids
337.326–9.024 14,15-DHET 1.702 1.557 0.029 Arachidonic acid metabolism; Serotonergic synapse
283.262–9.238 Octadecanoic acid 1.134 1.207 0.007 Fatty acid biosynthesis; Biosynthesis of unsaturated fatty acids
  1. Abbreviations: m/z - RT, MS and retention time; VIP, variable importance for projection; FC, fold change; q-value, adjusted P-value calculated by two-tailed Wilcoxon rank-sum tests after false discovery rate correction