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Fig. 4 | Parasites & Vectors

Fig. 4

From: Identification of Anaplasma ovis appendage-associated protein (AAAP) for development of an indirect ELISA and its application

Fig. 4

Specificity and assessment of cross-reactivity of recombinant AAAP using Western blot. Lane M: molecular weight marker; Lanes 1–11 had 5 μg of purified rAAAP blotted, which was then reacted with the primary antibodies as follows: Lane 1: mouse anti-RGS-His antibody; Lane 2: A. ovis-positive sheep serum; Lane 3: serum from uninfected sheep; Lane 4–9, 11: positive sheep sera against Mycoplasma ovipneumoniae, M. capricolum capricolum, Babesia motasi, Babesia sp. Xinjiang, Theileria uilenbergi, T. luwenshuni and A. bovis, respectively; Lane 10: positive cattle serum against A. marginale

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