Table 1 Comparing of semi-quantitative scoring of sampled fish tissues based on HE staining, calcofluor white staining and real-time PCR Ct-values
Pathology scores of each fish | Fish ID | Histopathology scores | Calcofluor staining scores | Real-Time PCR Ct-values | ||||
|---|---|---|---|---|---|---|---|---|
Gill Microvesicles | PGI | Peritonitis | Gill | Peritoneum | Heart/Kidney | Gill | ||
Acute | A6 | 3.0 | 1.0 | 2.0 | 3.0 | 2.0 | 17 | nd |
Acute | A1 | 3.0 | 1.0 | 1.0 | 2.0 | 2.5 | 17 | nd |
Acute | A3 | 3.0 | 1.0 | 2.0 | 3.0 | 1.5 | 17 | nd |
Acute | A7 | 3.0 | 1.0 | 3.0 | 2.0 | 3.0 | 18 | nd |
Acute | A4 | 3.0 | 1.0 | 1.5 | 3.0 | 1.0 | 20 | nd |
Acute | B1 | 3.0 | 1.0 | 2.5 | 3.0 | 1.0 | nd | 20 |
Subacute | B2 | 3.0 | 2.0 | 1.5 | 3.0 | 1.0 | nd | 22 |
Subacute | A5 | 2.0 | 1.0 | 3.0 | 1.0 | 1.5 | 24 | nd |
Subacute | B3 | 2.0 | 2.0 | 3.0 | 1.5 | 3.0 | nd | 27 |
Subacute | A2 | 1.0 | 1.0 | 3.0 | 1.0 | 0.5 | nd | nd |
Chronic | C2 | 2.0 | 2.5 | 1.5 | 2.0 | 1.0 | 21 | 20 |
Chronic | B4 | 2.0 | 3.0 | 2.5 | 2.5 | 2.0 | nd | 23 |
Chronic | C4 | 1.0 | 2.0 | 3.0 | 1.0 | 1.5 | 26 | 24 |
Chronic | C3 | 1.5 | 2.0 | 2.0 | 1.0 | 1.0 | 28 | 25 |
Chronic | B8 | 2.0 | 1.0 | 2.0 | 1.0 | 1.5 | nd | 26 |
Chronic | C1 | 1.0 | 0.5 | 1.5 | 1.0 | 0.0 | 25 | 26 |
Chronic | C6 | 1.0 | 2.0 | 1.5 | 0.0 | 0.0 | 23 | 27 |
Chronic | B7 | 1.0 | 0.5 | 3.0 | 1.0 | 2.5 | nd | 28 |
Chronic | B6 | 1.0 | 0.5 | 2.0 | 1.0 | 0.0 | nd | 30 |
Chronic | B5 | 1.0 | 0.5 | 2.0 | 1.0 | 1.0 | nd | 30 |
Chronic | C5 | 1.0 | 1.0 | 2.0 | 1.0 | 1.5 | 26 | 31 |