Fig. 12From: Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix Validation of the RNA-Seq results by quantitative real-time PCR (qRT-PCR). Nine unigenes with significant differences and one unigene without significant difference were randomly selected for analysis using qRT-PCR. GAPDH was used for normalization. Asterisks represent significant difference (PÂ <Â 0.05)Back to article page