Fig. 2
Assaying distinct T. cruzi strains/clones and triatomine species in the conventional multiplex PCR. a Reconstituted DNA samples (R. prolixus + T. cruzi DNAs) were used for the detection of distinct T. cruzi strains/clones with 121/122 primers (kDNA) in the multiplex format. Lane M: 100 bp DNA molecular marker; Lanes 1, 2: negative PCR controls (PCR reagents without DNA and ultra-pure water, respectively); Lane 3: DNA extraction negative control (extraction reagents + ultra-pure water); Lane 4: non-infected triatomine control (R. prolixus from insectary); Lanes 5, 6: DNA from R. prolixus midgut mixed with T. cruzi CL Brener (TcVI) DNA; Lanes 7, 8: DNA from R. prolixus midgut mixed with T. cruzi Dm28c (TcI) DNA; Lanes 9, 10: DNA from R. prolixus midgut mixed with T. cruzi INPA 4167 (TcIV) DNA; Lane 11: T. cruzi positive control (DNA obtained from 102 CL Brener epimastigotes); Lane 12: T. rangeli positive control (DNA obtained from 102 Macias strain epimastigotes). b Detection of distinct triatomine species using P2B/P6R primers (12S rRNA gene). Five individual midguts of each species were used as DNA source. Lane M: 100 bp DNA molecular marker; Lanes 1, 2: negative PCR controls (PCR reagents without DNA and ultra-pure water, respectively); Lane 3: DNA extraction negative control (extraction reagents in ultrapure water); Lanes 4, 5: R. neglectus; Lanes 6, 7: T. costalimai; Lane 8: T. pseudomaculata; Lane 9: T. wygodizinskyi; Lane 10: T. sordida; Lanes 11, 12: R. prolixus