Fig. 7

Comparative immunohistochemistry of PKD on rainbow trout kidney and gills during single and co-infection with WD. a, b Kidney sections of single Tb infected and Tb-then Mc co-infected groups respectively at 90 dpe showing moderate numbers of interstitial extrasporogonic stages of Tb (arrow). c, d Mc-then Tb co-infected group, 120 dp initial exposure to Mc (90 dpe to secondary Tb), (c) kidney section showing large number of interstitial extrasporogonic stages of Tb distributed in most of kidney interstitium (arrow), (d) gill section showing presence of one parasite stage of T. bryosalmonae in lamellar epithelium (arrow). Scale-bars: a, 100 μm (inset 50 μm); b, 100 μm (inset 20 μm); c, 100 μm (inset 20 μm); d, 20 μm