Fig. 1
GISH results using Triatoma rubrofasciata genomic DNA (gDNA) probe (labelled in yellow-green) on male chromosomes of different Triatoma species (labelled in red). a Self-GISH on own chromosomes of T. rubrofasciata (2n = 22A + X1X2Y): Second meiotic metaphase. Hybridization signals appear scattered on all chromatin, but the strongest signals are preferably located at the autosomal chromosome ends plus the Y chromosome. Both X chromosomes did not present hybridization signals. b T. rubrofasciata. First meiotic metaphase (MI) with C-banding. Heterochromatic regions with the same distribution pattern as observed with self-GISH. c T. dimidiata (2n = 20A + X1X2Y). Early meiotic prophase. d T. lecticularia (2n = 20A + XY): MI. e T. nitida (2n = 18A + X1X2Y): MI. In (c) to (e), strong hybridization signals are restricted to the heterochromatic Y chromosome. f T. nitida: MI with C-banding. The two C-heterochromatic bivalents did not exhibit hybridization signals (compare chromosomes pointed out with arrows in e and f). g T. barberi (2n = 20A + X1X2Y): MI. Y chromosome and one of the X chromosomes (X1) appear with strong signals. h T. (Mepraia) spinolai (2n = 20A + X1X2Y): MI. Only the Y chromosome shows hybridization signals. In (c) to (h), autosomal C-heterochromatic regions appear labelling free. i T. infestans (2n = 20A + XY): Spermatogonial prometaphase. Strong hybridization signals are observed on the Y chromosome and five autosomes. Scale-bars: 5 μm. Abbreviations: A, autosomes; MI, metaphase I