Skip to main content
Fig. 2 | Parasites & Vectors

Fig. 2

From: The N- and C-terminal carbohydrate recognition domains of Haemonchus contortus galectin bind to distinct receptors of goat PBMC and contribute differently to its immunomodulatory functions in host-parasite interactions

Fig. 2

Testing protein-protein interaction of MNh to TMEM63A or TMEM147 and the interaction of MCh to TMEM63A or TMEM147 using DUAL membrane pairwise interaction assay. a Cells grown on control SD-LW block (without Leu and Trp) medium. b Cells grown on selective SD-AHLW block (without Ade, His, Leu and Trp). Yeast strain NMY51 carried each pairs of bait and prey plasmids (pBT3-STE, pBT3-SUC and pPR3-N are the control vectors with no cloned cDNA). The construct pairs of TMEM63A with pPR3-N, MNh with pBT3-STE, MCh with pBT3-STE, TMEM147 with pPR3-N, MNh with pBT3-SUC and MCh with pBT3-SUC were used as negative controls. The construct pairs of TMEM63A with MNh, TMEM63A with MCh, TMEM147 with MNh and TMEM147 with MCh were used as positive controls

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us