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Table 1 Details of the primer sequences and combinations evaluated for the assay development

From: SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Primer name

Sequence (5′-3′)

Genome positiona

Forward primers

 ZIKV-Fb

TTGGTCATGATACTGCTGATTGC

941–963

 ZIKV-F2

TCAACGAGCCAAAAAGTCAT

917–936

 ZIKV-F3

ATCAGGTGCATAGGAGTCAG

977–996

Reverse primers

 ZIKV-Rb

CCTTCCACAAAGTCCCTATTGC

996–1016

 ZIKV-R3

CAGGTCCCACCTGACATGC

1017–1035

 ZIKV-R4

CAACGACTGTCCGAAGCCATG

1177–1197

  1. aGenome positions were calculated from the first base of the 5′ non-translated region of the ZIKV reference sequence in GenBank nucleotide database (Accession no. NC012532.1)
  2. bPrimers previously used for a probe-based rRT-PCR protocol published by Lanciotti et al. [9]
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Parasites & Vectors

ISSN: 1756-3305

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