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Table 1 Characteristics of the HRM qPCR assay performances

From: Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis

Parameter PCR assay
HRM qPCR for ITS1 HRM qPCR for 18S HRM qPCR for cytb
Efficiency (%) 90 103 134
Slope -3.61 -3.23 -2.70
y-intercept 35.15 34.88 40.12
R2 value 0.9976 0.9962 0.9896