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Table 1 Characteristics of the HRM qPCR assay performances

From: Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis

Parameter

PCR assay

HRM qPCR for ITS1

HRM qPCR for 18S

HRM qPCR for cytb

Efficiency (%)

90

103

134

Slope

-3.61

-3.23

-2.70

y-intercept

35.15

34.88

40.12

R2 value

0.9976

0.9962

0.9896

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Parasites & Vectors

ISSN: 1756-3305

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