Fig. 2

TvCMD1 does not participate in β-ketoadipate degradation pathway. a Aromatic compounds induction assay in T. vaginalis. Western blot was used to monitor the expression of TvCMD1 after incubating with protocatechuate (Pca) and benzoate (Ben) for 3 h. GAPDH was used as the loading control. b, c The detection of aromatic compounds in trichomonad cell lysates. The parent and product ions (m/z) ratio was defined according to multiple reaction monitoring (MRM) of each standard. The quantification of aromatic compounds (protocatechuate, benzoate) in iron-rich (middle panel, IR) and iron-deficient (upper panel, ID) cultured T. vaginalis. Percentage (Y-axis) represents the relative concentration of each compound. The lower panels are the standards (std, 1 ppm) Pca and Ben. The protein induction assay and metabolites detection showed that TvCMD1 is unlikely to catalyze aromatic compounds protocatechuate and benzoate