Fig. 4

Effect of L. major and L. tropica SLA on NO production by macrophages. The J774 macrophages cells were cultured in presence of L. tropica SLA (a), L. major SLA (b), and stimulated according to the same protocol than promastigotes. The supernatants were collected and nitrite concentrations were evaluated by Griess reaction after 24 h post-infection. Results are expressed as mean ± SEM from three independent experiments, each performed in duplicate. Data were analyzed using one way ANOVA test with Tukey’s post-hoc test for multiple comparisons. ***P < 0.001