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Fig. 3 | Parasites & Vectors

Fig. 3

From: Screening and identification of host proteins interacting with Theileria annulata cysteine proteinase (TaCP) by yeast-two-hybrid system

Fig. 3

Expression, auto-activation and toxicity tests for pGBKT7-TaCP bait. a Western blot analysis of total protein extracts of Y2HGold. Lane 1: pGBKT7-TaCP; Lane 2: pGBKT7-53. b Determination of the auto-activation and toxicity activity of the pGBKT7-TaCP bait plasmid in yeast cells. The pGBKT7-TaCP bait and pGBKT7 plasmids were used to transform Y2HGold cells and then grown on different plates. The co-transformants containing pGADT7-T and pGBKT7-53 were grown on DDO/X/A plates as positive control

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Parasites & Vectors

ISSN: 1756-3305

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