Table 1 Primers and probe used for PCR testing
From: Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain
Oligonucleotide | Type | Sequence (5′–3′) |
|---|---|---|
BsppITS325s | Sense primer | CCTCAGATGATGATCCCAAGCCTTCTGGCG |
BsppITS543as | Antisense primer | AATTGGTGGGCCTGGGAGGACTTG |
BsppITS1100as | Antisense primer | GAACCGACGACCCCCTGCTTGCAAAGCA |
BsppITS438 | Taqman probe | FAM-AGGTTTTCC/ZEN/GGTTTATCCCGGAGGGC-IABkFQ |
Bkoehl-1s | Sense primer | CTTCTAAAATATCGCTTCTAAAAATTGGCATGC |
Bkoehl1125as | Antisense primer | GCCTTTTTTGGTGACAAGCACTTTTCTTAAG |