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Table 1 Effects of Aedes albopictus larval rearing densities on the pupae production and larval survival at different pupation times when using the Wol-tray or the IAEA-tray

From: Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)

Tray

Number of larvae per tray

34 h (Mean ± SE)

58 h (Mean ± SE)

Larval survival (%)a (Mean ± SE)

PP

MPP

M%

PP

MPP

M%

Wol-tray

5300

3092 ± 27 a

2396 ± 46 a

70.0 ± 1.9 a

1252 ± 60 a

358 ± 53 a

25.6 ± 2.8 ab

95.2 ± 2.4 a

6600

3986 ± 62 b

3003 ± 29 b

72.8 ± 0.5 a

1798 ± 15 b

345 ± 22 a

18.5 ± 0.9 a

99.0 ± 0.4 a

7900

4152 ± 22 b

3188 ± 98 b

74.2 ± 1.1 a

2312 ± 10 c

662 ± 19 b

27.7 ± 1.1 b

96.1 ± 1.0 a

IAEA-tray

12,000

6752 ± 192 A

5238 ± 63 A

76.3 ± 0.4 A

3306 ± 196 A

712 ± 60 A

21.1 ± 0.7 A

97.9 ± 1.0 A

15,000

8240 ± 31 B

5961 ± 124 A

71.1 ± 0.6 B

4540 ± 77 B

937 ± 52 A

20.2 ± 0.4 A

98.4 ± 0.8 A

18,000

10,281 ± 125 C

7349 ± 346 B

70.2 ± 1.6 B

4335 ± 78 B

883 ± 76 A

20.0 ± 1.1 A

97.7 ± 1.0 A

  1. Abbreviations: PP pupae production, MPP male pupae production, M% percentage of male pupae in PP
  2. aLarval survival was calculated as: (Total number of pupae collected + Residual number of larvae after sex separation) / (Estimated number of larvae per tray)
  3. Note: Within each column, values followed by different lowercase or capital letters were statistically different using ANOVA analysis and Tukey post-hoc test (P < 0.05)
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