Fig. 4
From: Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity
Analysis of the rLbSCD6 and rLbRBP42 binding capacity to the 3′ UTR-II.1 motif. a RNA secondary structure of the 3′ UTR-II.1 motif; the putative binding sequence is underlined. The structure motif was obtained by RNAfold. EMSA assays were done using a stable concentration of rLbrSCD6 (b) and rLbRBP42 (c) proteins. Different mixtures of both labeled and cold 3′ UTR-II.1 motif were used. These mixtures contained a constant concentration of the labeled ribonucleotide and different amounts (1-, 2-, 4- or 8-fold excess) of the non-labeled (cold) ribonucleotide (Lanes 6–9). In lane 5, the reaction was done only in the presence of the 3′ UTR-II.1-dig ribonucleotide. As control of unspecific binding, the denatured recombinant proteins were used